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US 2022/0178826 A1Published application
Method and system for visualization of endotoxins in a fluid sample
AssigneeBl Technologies, Inc.
InventorsSukmi LEDRU, Krzysztof FRANASZCZUK, Jesse MANLEY, David KREMER, Vamsi PANUGANTI, Jeremiah TEAGUE, Yan LE, Michael SCAER
Life of this patent
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Abstract
Exemplified methods and systems facilitate presentation of data derived from measurements of endotoxins in fluid samples. In particular, the exemplified methods and systems facilitate presentation of such measurements in a graphical user interface and/or in a report for endotoxin concentrations in a fluid sample. The presentation facilitates a unified and intuitive graphic visualization that are presented within a single interactive interface and/or report.
Claims35
A computer-implemented method to generate visualizations of endotoxin concentrations in a fluid sample, the method comprising:
- generating, by a processor, for a graphical user interface or for a report, to be displayed on a stationary or mobile computing device, a graphical visualization, from a data set that identifies an optical density (OD) reading of a microplate comprising a plurality of optical wells, each optical well containing a fluid sample, said graphical visualization comprising a vertically-oriented bar graph having a plurality of vertical bars with at least one vertically-oriented bar graph for each optical well of the microplate,
- wherein the processor causes the vertically-oriented bar graph corresponding to the optical well of the microplate to increase vertically over time as a reaction between Limulus Amoebocyte Lysate (LAL) and bacterial endotoxin takes place in the optical well of the microplate,
- wherein a greater amount of endotoxin present in the optical well, the faster the reaction occurs with the LAL reagent, and subsequently the faster the processor causes the associated vertically-oriented bar graph to increase on the graphical visualization.
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A system for testing of endotoxin in inject-able drug product or water, the system comprising:
- a processor;
- and
- a memory having instructions stored thereon, wherein execution of the instructions by the processor, cause the processor to:
- continuously obtain one or more data sets associated with measurement of a multi-well micro plate during acquisition of measurement of the multi-well micro plate, wherein each measurement is associated with a measured level of endotoxins reactions of i) endotoxins in an acquired sample of inject-able drug product or water to ii) one or more test reagents over time, and wherein the data set comprises a current measured level of endotoxin reactions for each well of the multi-well micro plate, wherein the multi-well micro plate comprises at least 96 wells;
- and
- singularly display, in a pane of a graphical user interface, from the obtained data set, a side-by-side graph comprising a plurality of visual elements, wherein each visual element of the plurality of visual elements is associated with the current measured level for the each well of the at least 96 wells of the multi-well micro plate,
- wherein each visual element of the plurality of visual elements comprises a vertically oriented bar that is configured to display data values spanning a range between a minimum value and a maximum value, wherein each of the minimum value and maximum value are aligned with a respective first and second horizontal line,
- wherein the display further comprises a visual element comprising a third horizontal line at a value that indicates an onset threshold of an endotoxin reaction for each, or among group of wells, of the at least 96 wells of the multi-well micro plate, and
- wherein the side-by-side graph of the at least 96 wells of the multi-well micro plate provides simultaneous comparison of a progress of endotoxin reaction in, or among group of wells of, the at least 96 wells of the multi-well micro plate to test endotoxin in the acquired sample of inject-able drug product or water.
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A method to monitor testing of endotoxin in inject-able drug product or water, the method comprising:
- obtaining, by a processor, one or more data sets associated with measurement of a multi-well micro plate, where each measurement is associated with a measured level of endotoxins in an acquired sample of inject-able drug product or water over time, wherein the data set comprises a current measured level for each well of the multi-well micro plate, wherein the multi-well micro plate comprises at least 96 wells;
- and
- singularly displaying, by the processor, in a pane of a graphical user interface, from the obtained data set, a side-by-side graph comprising a plurality of visual elements, wherein each visual element of the plurality of visual elements is associated with a current measured level of each of the at least 96 wells of the multi-well micro plate,
- wherein each visual element of the plurality of visual elements comprises a vertically oriented bar that is configured to span data values ranging between a minimum and a maximum value, wherein each of the minimum value and maximum value are coincident with a first and second horizontal line,
- wherein the display further comprises a visual element comprising a third horizontal line at a measured value that indicates onset threshold for each of the at least 96 wells of the multi-well micro plate, and
- wherein the side-by-side graph of the at least 96 wells of the multi-well micro plate provides simultaneous comparison of speed of reaction in, or among group of wells of, the at least 96 wells of the multi-well micro plate to monitor testing of endotoxin in the acquired sample of inject-able drug product or water.
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A non-transitory computer readable medium having instructions stored thereon, wherein execution of the instructions by a processor, cause the processor to:
- obtain one or more data sets associated with measurement of a multi-well micro plate, where each measurement is associated with a measured level of endotoxins in an acquired sample of inject-able drug product or water over time, wherein the data set comprises a current measured level for each well of the multi-well micro plate, wherein the multi-well micro plate comprises at least 96 wells;
- and
- singularly display, in a pane of a graphical user interface, from the obtained data set, a side-by-side graph comprising a plurality of visual elements, wherein each visual element of the plurality of visual elements is associated with a current measured level of each of the at least 96 wells of the multi-well micro plate,
- wherein each visual element of the plurality of visual elements comprises a vertically oriented bar that is configured to span data values ranging between a minimum and a maximum value, wherein each of the minimum value and maximum value are coincident with a first and second horizontal line,
- wherein the display further comprises a visual element comprising a third horizontal line at a measured value that indicates onset threshold for each of the at least 96 wells of the multi-well micro plate, and
- wherein the side-by-side graph of the at least 96 wells of the multi-well micro plate provides simultaneous comparison of speed of reaction in, or among group of wells of, the at least 96 wells of the multi-well micro plate to monitor testing of endotoxin in the acquired sample of inject-able drug product or water.
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Dependencies are inferred from the claim text.