Apparatus and method for determination of banned substances

An apparatus for the separation and determination of regulated compounds in biological fluid sample using electrophoresis and comprising

• at least one separation capillary;
• at least one fluorescence detector for characterizing electrophoretic zones of compounds passing through a detection zone of the at least one separation capillary;
• an injection system for introducing fluids, including sample solutions and background electrolyte into an inlet end of the at least one separation capillary, to conduct a sample processing sequence prior to a sample analysis sequence;
• a high voltage power supply;
• a computerized system for commanding the injection system, flow of fluids through the at least one separation capillary, and operation of the at least one fluorescence detector;
• a sample preparation and extraction device for processing a biological fluid sample, the device comprising a compartment for swab/pad/tampon comprising the biological fluid sample, vials for collecting excess oral fluid and sample solution, solenoid valves for controlling transport of the biological fluid sample through the device vessel containing extractant, a computerized controlling system for commanding the solenoid valves that facilitate flow of fluids through the separation channel, to conduct the sample processing sequence;
• the apparatus being configured to conduct the sample analysis sequence after conducting the sample processing sequence, wherein the sample analysis sequence comprises introduction of a sample and a background electrolyte and applying a voltage potential across the at least one separation capillary to effect separation of the compounds.