Opto-Fluidic Apparatus for Individual Interrogation of Organisms

Abstract

The present invention relates to an apparatus for the detection of optical emission from individual members of a sample of micro-organisms where the flow is focused in a specific region, comprising an optical device including a light source configured to emit light in a first direction to individual members of the sample and a detection device configured to detect in a field of view optical emission from the individual members emitted in a second direction opposite to the first direction and excited by the light emitted by the light source and a fluid device including a storage reservoir configured to store the sample of micro-organisms, a pumping device, in particular, a circulation pump capable of producing different pulsed pressures to affect the flow configured to circulate the sample of micro organisms in the storage reservoir and wherein a capillary channel is arranged in the storage reservoir configured to allow a flow of individual members of the sample such that one individual member only is present in the field of view of the detection device at a given time of detection of the optical emission.

Dependencies are inferred from the claim text.