Apparatuses and methods for rapid collection of microbe from a sample
AssigneePathOtrak, LLC
InventorsFrancisco Javier ATENCIA-FERNANDEZ, Ethan REGGIA, Drew TACK
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Abstract
The present invention provides apparatuses and methods for collecting microbes in a sample. The present invention provides apparatuses, systems, or devices, configured to receive a test sample, comprising one or more sealable containers configured to receive a test sample comprising a liquid portion, at least one outlet port, and comprising at least one filtration filter and at least one concentration filter fluidly connected in sequence to the at least one outlet port to form a filtered outlet port.
Claims32
An apparatus, comprising:
- one or more sealable containers configured to receive a test sample comprising a liquid portion,
- at least one outlet port, and
- at least one filtration filter and at least one concentration filter fluidly connected in sequence to the at least one outlet port to form a filtered outlet port,
- wherein after the one or more sealable containers is sealed, the apparatus is configured to permit flow of the liquid portion of the test sample from the one or more sealable containers through the at least one outlet port into and through the filtered outlet port when a pressure difference of equal to or greater than about 4 pounds per square inch (psi) exists between the one or more sealable containers and the filtered outlet port,
- wherein the test sample comprises or is suspected to comprise a microbe, and
- wherein each of the at least one filtration filter and the at least one concentration filter has a pore size equal to or greater than the average size of the microbe.
A method of concentrating a microbe from a test sample comprising a liquid portion, comprising:
- (1) passing the test sample through an apparatus comprising one or more sealable containers configured to receive the test sample comprising the liquid portion, at least one outlet port, and at least one filtration filter and at least one concentration filter fluidly connected in sequence to the at least one outlet port to form a filtered outlet port, wherein after the one or more sealable containers is sealed, the apparatus is configured to permit flow of the liquid portion of the test sample from the one or more sealable containers through the at least one outlet port into and through the filtered outlet port when a pressure difference of equal to or greater than about 4 pounds per square inch (psi) exists between the one or more sealable containers and the filtered outlet port, and wherein each of the at least one filtration filter and the at least one concentration filter has a pore size equal to or greater than the average size of the microbe;
- and
- (2) collecting a concentrated liquid portion of the test sample from the at least one concentration filter, the concentrated liquid portion of the test sample including the microbe.
Dependencies are inferred from the claim text.