Polycationic quaternary ammonium polymer coatings for immobilizing biological samples
AssigneeTriPath Imaging, Inc.
InventorsWilliam A. Fox, William Carl Ray, III
Life of this patent
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Abstract
The present invention is directed to a pre-coated substrate, such as a slide, that is useful for immobilizing a sample. The invention is further provides methods of preparing such pre-coated substrates and methods of analyzing biological samples immobilized on such pre-coated substrate. The substrate is coated with a polycationic polymeric coating material specifically selected such that that coated substrate exhibits increased stability and prolonged shelf-life. Preferred polymeric coating materials include allylic or vinylic polymers having cationic groups thereon and having no more than a small percentage of peptidic monomeric linkages, particularly polydiallyldimethylammonium (PDDA).
Claims21
-48. (canceled)
A coated substrate for immobilizing a biological sample for analysis, comprising:
- a substrate, wherein the substrate is a glass slide;
- and
- a coating comprising a non-peptidic quaternary ammonium polymeric material having an average molecular weight greater than 350,000 Da, wherein the coating allows cells in a biological sample to be immobilized directly thereon when brought into contact with the biological sample.
The coated substrate of clam 62, wherein the solution has a pH of about 9.2.
Dependencies are inferred from the claim text.