Fluorogenic nucleic acid molecule and target rna fluorescent labeling method
AssigneeJapan Science and Technology Agency
InventorsYasushi OKADA, Tetsuro ARIYOSHI
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Abstract
The present invention provides a fluorogenic RNA capable of visualizing mRNA in cells, particularly, living mammalian cells, and a target RNA fluorescent labeling method using the fluorogenic RNA. The present invention provides a fluorogenic nucleic acid molecule containing a base sequence having two or more fluorescent molecule-binding regions linked via a linker sequence, in which one or more fluorescent molecule-binding aptamer sequences are inserted into a scaffold sequence of each of the fluorescent molecule-binding regions.
Claims21
A fluorogenic nucleic acid molecule, comprising:
- a base sequence having 2 or more fluorescent molecule-binding regions linked via a linker sequence,
- wherein 1 or more fluorescent molecule-binding aptamer sequences are inserted into a scaffold sequence of each of the fluorescent molecule-binding regions.
A linker sequence-designing method that is a method of designing a linker sequence in a fluorogenic nucleic acid molecule containing a base sequence having 2 or more fluorescent molecule-binding regions which each have 1 or more fluorescent molecule-binding aptamer sequences inserted into a scaffold sequence and are linked via the linker sequence, the method comprising:
- designing a linker sequence so that in a case where a candidate linker sequence is linked to upstream and downstream side of each of the fluorescent molecule-binding regions to prepare an RNA sequence used as an RNA sequence for evaluation, a result of prediction of a 3-dimensional structure of the fluorescent molecule-binding region in the RNA sequence for evaluation is identical to a result of prediction of a 3-dimensional structure of an RNA sequence consisting only of the fluorescent molecule-binding region, and the candidate linker sequence portion is not predicted to have a 3-dimensional structure.
21-23. (canceled)
Dependencies are inferred from the claim text.