Method to detect and enumerate microorganisms
Assignee3M Innovative Properties Company
InventorEvan D. Brutinel
Life of this patent
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Abstract
A method. The method includes providing a device, the device comprising: a body member comprising a self-supporting, waterproof substrate having upper and lower surfaces; and a layer of adhesive-coated on the upper surface of the substrate, the adhesive being non-inhibitory to the growth of microorganisms; and a cold-water-soluble powder adhered to the adhesive, the powder comprising: guar gum; one or more nutrients for growing microorganisms; and a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the body member; incubating a sample in the device to form a gel; and counting colonies between 24 hours to 72 hours and/or determining if the gel surrounding a colony is degraded.
Claims14
A method, comprising
- providing a device, the device comprising:
- a body member comprising a self-supporting, waterproof substrate having upper and lower surfaces;
- and
- a layer of adhesive-coated on the upper surface of the substrate, the adhesive being non-inhibitory to the growth of microorganisms;
- and a cold-water-soluble powder adhered to the adhesive, the powder comprising:
- guar gum;
- one or more nutrients for growing microorganisms;
- and
- a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the body member;
- incubating a sample in the device to form a gel;
- and
- counting colonies between 24 hours to 72 hours and/or determining if the gel surrounding a colony is degraded.
Dependencies are inferred from the claim text.