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US 2022/0177940 A1Published application
Enzymatic method for preparation of gdp-fucose
AssigneeMax-Planck-Gesellschat Zur Forderung Der Wissenschaften E.V.
InventorsReza Mahour, Thomas F.T. Rexer
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Abstract
The present invention relates to an enzyme-catalyzed process for producing GDP- fucose from low-cost substrates guanosine and L-fucose or guanosine and D-Mannose in a single reaction mixture. Said process can be operated (semi)continuously or in batch mode. Further, said process can be adapted to produce fucosylated molecules and biomolecules including glycans, such as human milk oligosaccharides, proteins, peptides, glycoproteins or glycopeptides.
Claims19
A method for producing guanosine 5′-diphospho-β-L-fucose from guanosine and L-fucose or D-mannose comprising:
- A) providing a solution comprising
- (i) guanosine and L-fucose or guanosine and D-mannose represented by the following formulae
- (ii) polyphosphate, adenosine triphosphate and a co-solvent for solubilizing guanosine;
- and
- providing a set of enzymes comprising a guanosine kinase, a polyphosphate kinase, and in case of L-fucose a L-fucokinase/L-fucose- 1-phosphate guanylyltransferase or in case of D-mannose either (a) a glucokinase, phosphomannomutase, a mannose-1-phosphate guanylyltransferase, a GDP-mannose-4,6-dehydratase and a GDP-L-fucose-synthase or (b) an N-acetylhexosamine-1-kinase, a mannose-1-phosphate guanylyltransferase, a GDP-mannose 4,6-dehydratase and a GDP-L-fucose-synthase;
- B) producing guanosine 5′-diphospho-β-L-fucose from guanosine and L-fucose in the presence of the set of enzymes, polyphosphate, adenosine triphosphate and the co-solvent or from guanosine and D-mannose in the presence of the set of enzymes, polyphosphate, adenosine triphosphate and the co-solvent.
3 more elements
Dependencies are inferred from the claim text.