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US 2022/0177929 A1Published application
Biofixation of greenhouse gas by mass culture of haematococcus sp. kau-01 microalga in high efficiency medium
AssigneeKing Abdulaziz University
InventorsAdnan Jaman TURKI, Md. Abu AFFAN, Salim Marzoog AL-HARBI
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Abstract
The invention is directed to a culture medium for Haematococcus that contains combustion gases like carbon dioxide, carbon monoxide, and oxides of nitrogen or sulfur and which fixes the carbon, nitrogen or sulfur in these combustion gases into biomass and to methods providing superior biomass yields using this culture medium to culture select species of Haematococcus such as Haematococcus sp. KAU-01.
Claims20
A method for producing biomass, comprising:
- culturing Haematococcus in a culture medium in the presence at least one combustion gas and light, thereby producing biomass;
- wherein said culture medium comprises from:
- 187.50 to 562.50 mg/L NaNO3, 37.50 to 112.50 mg/L KNO3, 12.50 to 37.50 mg/L Ca(NO3)2, 27.50 to 82.50 mg/L Mg(NO3)2.6H2O, 22.50 to 67.50 mg/L K2HPO4, 20.00 to 60.00 mg/L KH2PO4, 5.00 to 15.00 mg/L K2SO4, 11.88 to 35.63 mg/L MgSO4.7H2O, 0.88 to 2.63 mg/L urea, 32.50 to 97.50 μl/L HNO3, 7.50 to 22.50 μl/L H3PO4, and freshwater.
A culture medium comprising from:
- 187.50 to 562.50 mg/L NaNO3, 12.50 to 37.50 mg/L Ca(NO3)2, 27.50 to 82.50 mg/L Mg(NO3)2.6H2O, 22.50 to 67.50 mg/L K2HPO4, 20.00 to 60.00 mg/L KH2PO4, 37.50 to 112.50 mg/L KNO3, 5.00 to 15.00 mg/L K2SO4, 11.88 to 35.63 mg/L MgSO4.7H2O, 0.88 to 2.63 mg/L urea, 32.50 to 97.50 μl/L HNO3, 7.50 to 22.50 μl/L H3PO4;
- 1.75 to 5.25 mg/L FeCl3.6H2O, 0.50 to 1.50 mg/L H3BO3, 0.13 to 0.38 mg/L Co(NO3)2.6H2O, 0.05 to 0.15 mg/L K2Cr2O7, 0.05 to 0.15 mg/L CuSO4.5H2O, 0.13 to 0.38 mg/L MnSO4.H2O, 0.38 to 1.13 mg/L ZnSO4.6H2O, 0.13 to 0.38 mg/L (NH4)6Mo7O24.4H2O, 0.5 to 1.5 mg/L Na2-EDTA, and 0.500 to 1.50 μl/L HCl;
- and
- further comprises 0.8 to 1.2 mg/L thiamine, 0.0024 to 0.0036 mg/L+D-biotin, and 0.0008 to 0.0012 mg/L cyanocobalamin.
1 more element
Dependencies are inferred from the claim text.