Genome editing system

A system for editing of a target sequence at a locus of a host cell, comprising:

• i) a first isolated nucleic acid molecule comprising a nucleic acid segment comprising or encoding a targeting RNA sequence;
• ii) a second isolated nucleic acid molecule comprising a nucleic acid segment comprising or encoding a RNA segment that binds a protein having at least 80% amino acid sequence identity to SEQ ID NO:5;
• iii) a third isolated nucleic acid molecule comprising a nucleic acid segment encoding an endonuclease or a portion thereof with nuclease activity, or an isolated polypeptide comprising the endonuclease or the portion thereof;
• iv) a fourth isolated nucleic acid molecule comprising a nucleic acid segment encoding the protein having at least 80% amino acid sequence identity to SEQ ID NO:5 or a portion thereof that binds the RNA segment, or an isolated polypeptide comprising the protein having at least 80% amino acid sequence identity to SEQ ID NO:5 or the portion thereof that binds the RNA segment;
• and
• v) an isolated double stranded DNA molecule comprising DNA comprising at least one nucleotide sequence that is capable of binding to the target sequence at the locus.