Loading patent…
AssigneeJiangnan University
InventorsSong Liu, Cen Li, Guocheng Du, Jian Chen, Jingwen Zhou
Estimating the term…
The present disclosure discloses a visualized screening method for an Aspergillus recombinant strain with multigene editing and belongs to the technical field of gene engineering. CRISPR-Cas9 is used in the disclosure to cleave spore color change-related genes and a target gene in Aspergillus at the same time, such that editing of the target gene is visualized and an Aspergillus niger strain with multigene editing can be rapidly and efficiently screened out through spore phenotypes. Through different combinations of visualized genes and non-phenotypic change genes, rapid screening of the strain with multigene editing and simultaneous screening of multiple visualized genes are realized, and use of resistance genes in industrial strains is reduced.
A method for visualized screening of an Aspergillus strain with gene editing, wherein using CRISPR-Cas9 gene editing technology to simultaneously knock out genes (a) and (b) in the Aspergillus;
A visualized system for gene knockout in Aspergillus, wherein the visualized system comprises a gene encoding a Cas9 protein, an sgRNA expression cassette and a screening marker;
Dependencies are inferred from the claim text.