US 2022/0177924 A1 · GENETICALLY MODIFIED MICROORGANISM AND METHOD FOR PRODUCING TARGET SUBSTANCE USING SAME

Abstract

The present disclosure relates to a genetically modified microorganism satisfying some of predetermined conditions. The predetermined conditions include: (I) succinate dehydrogenase activity or fumarate reductase activity being reduced or inactivated relative to a wild-type microorganism; (II) lactate dehydrogenase activity being reduced or inactivated relative to the wild-type microorganism; (III) the genetically modified microorganism having modified phosphoenolpyruvate carboxylase activity showing resistance to feedback inhibition by aspartic acid in wild-type phosphoenolpyruvate carboxylase activity, or exogenous phosphoenolpyruvate carboxylase activity having higher resistance to feedback inhibition by aspartic acid than that of the wild-type phosphoenolpyruvate carboxylase activity shown by the wild-type microorganism; and (IV) pyruvate:quinone oxidoreductase being reduced or inactivated relative to the wild-type microorganism.

Claims26

Dependent claims

A genetically modified microorganism satisfying all of the following Conditions (I) to (III):

Condition (I):
succinate dehydrogenase activity or fumarate reductase activity is reduced or inactivated relative to a wild-type microorganism corresponding to the genetically modified microorganism;
Condition (II):
lactate dehydrogenase activity is reduced or inactivated relative to the wild-type microorganism;
and
Condition (III):
the genetically modified microorganism has modified phosphoenolpyruvate carboxylase activity showing resistance to feedback inhibition by aspartic acid in wild-type phosphoenolpyruvate carboxylase activity, or exogenous phosphoenolpyruvate carboxylase activity having higher resistance to feedback inhibition by aspartic acid than that of the wild-type phosphoenolpyruvate carboxylase activity shown by the wild-type microorganism.

4 more elements

A mutant-type phosphoenolpyruvate carboxylase comprising an amino acid mutation with respect to an amino acid sequence of a wild-type phosphoenolpyruvate carboxylase of a microorganism belonging to coryneform bacteria, the amino acid mutation being capable of reducing feedback inhibition by aspartic acid in the wild-type phosphoenolpyruvate carboxylase activity,

wherein the amino acid mutation at least comprises:
based on the amino acid sequence set forth in SEQ ID NO:
2,
(g) an amino acid substitution of an amino acid corresponding to the 299th aspartic acid with asparagine;
(i) an amino acid substitution of an amino acid corresponding to the 813th lysine with a predetermined amino acid, wherein the substituted amino acid is not lysine;
or
(l) an amino acid substitution of an amino acid corresponding to the 917th asparagine with a predetermined amino acid, wherein the substituted amino acid is not asparagine, wherein
the mutant-type phosphoenolpyruvate carboxylase has higher resistance to feedback inhibition by aspartic acid than that of a protein having only the amino acid substitution defined in (g), (i), or (l) above with respect to the amino acid sequence of the wild-type phosphoenolpyruvate carboxylase.

5 more elements

Dependencies are inferred from the claim text.

Genetically modified microorganism and method for producing target substance using same

Life of this patent

Genetically modified microorganism and method for producing target substance using same

Life of this patent

Abstract

Claims26