Inducible expression from transposon-based vectors and uses

A recombinant expression vector, comprising:

• (a) a first expression cassette, comprising:
• (i) a control sequence comprising:
• (A) a murine cytomegalovirus (mCMV) enhancer sequence, comprising a mCMV enhancer element (mCMV-E) and a CMV promoter (CMV-P) sequence at its 3′ end, operably linked 5′ to a rat EF-1alpha intron sequence, comprising one or more TetO sequences inserted within the CMV-P sequence;
• (B) an intervening first leader sequence operably linked, 3′ to the CMV-P sequence of the mCMV enhancer sequence, and 5′ to the rat EF-1alpha intron sequence;
• and
• (C) a second leader sequence operably linked 3′ to the rat EF-1alpha intron sequence;
• (ii) an open reading frame encoding a first immunoglobulin subunit operably linked to the control sequence;
• and
• (iii) a first polyadenylation site operably linked 3′ to the open reading frame encoding the first immunoglobulin subunit;
• (b) a second expression cassette 3′ to the first expression cassette, comprising:
• (i) a control sequence comprising a promoter;
• (ii) an open reading frame encoding a second immunoglobulin subunit operably linked to the promoter;
• and
• (iii) a second polyadenylation site operably linked 3′ to the open reading frame encoding the second immunoglobulin subunit;
• and
• (c) a transcription termination sequence 3′ to the first expression cassette and 5′ to the second expression cassette.