US 2022/0177912 A1 · THERAPEUTIC PARTICLES THAT ENABLE ANTIGEN PRESENTING CELLS TO ATTACK CANCER CELLS

Abstract

Embodiments of the present disclosure pertain to modified antigen presenting cells that include a recombinant protein appended onto a surface of the antigen presenting cells. The recombinant protein can include: an ectodomain positioned on the surface; a transmembrane domain with a region embedded in the cell membrane; an antigen presenting cell recruiting domain that directs the cells towards the cancer cells; and an antigen presenting cell activator that activates or licenses the antigen presenting cells. Additional embodiments of the present disclosure pertain to methods of expressing the recombinant proteins on antigen presenting cells and utilizing the modified antigen presenting cells for treating various cancers in various subjects. Further embodiments of the present disclosure pertain to nucleotide-containing carriers for expressing the recombinant proteins of the present disclosure in antigen presenting cells.

Claims30

Dependent claims

A method of expressing a recombinant protein on antigen presenting cells, said method comprising:

introducing a carrier into the antigen presenting cells,
wherein the carrier comprises a nucleotide sequence expressing the recombinant protein,
wherein the recombinant protein is expressed by the antigen presenting cells from the nucleotide sequence and appended onto a surface of the antigen presenting cells, and
wherein the recombinant protein comprises:
an ectodomain that is positioned on the surface of the antigen presenting cells, wherein the ectodomain is capable of preferentially binding to an antigen of cancer cells, and
a transmembrane domain comprising at least one region embedded in the antigen presenting cell membrane,
wherein the transmembrane domain comprises a glycosylphosphatidylinositol (GPI)-anchored domain, and
wherein the transmembrane domain serves as an anchor for maintaining the ectodomain of the recombinant protein on the surface of the antigen presenting cells.

5 more elements

Dependencies are inferred from the claim text.

Therapeutic particles that enable antigen presenting cells to attack cancer cells

Life of this patent

Therapeutic particles that enable antigen presenting cells to attack cancer cells

Life of this patent

Abstract

Claims30