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AssigneeSuzhou Curemed Biomedical Technology Co. Ltd.
InventorsZhenhua SUN, Chijian ZUO, Jiafeng ZHU
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The present disclosure relates to a recombinant nucleic acid molecule of the transcriptional circular RNA and its application in protein expression. Specifically, the present disclosure relates to a recombinant nucleic acid molecule of the transcriptional circular RNA, recombinant expression vector, pre-circularized RNA, circular RNA, recombinant host cell, pharmaceutical composition and protein preparing method. The transcription product of the recombinant nucleic acid molecule in this present disclosure is a circular RNA which containing specific IRES element. IRES element can increase the protein expression level of circular RNA in eukaryotic cells, achieve efficient and persistent expression of protein. It has important application value in many fields like: Preparation of mRNA infectious disease vaccines, therapeutic mRNA tumor vaccines, mRNA-based dendritic cell tumor vaccines, mRNA-based gene therapy, mRNA-based chimeric antigen receptor T cell therapy, and protein supplement therapy.
A recombinant nucleic acid molecule for making circular RNA, the recombinant nucleic acid molecule comprising, from the 5′ end to the 3′ end, a 3′ intron, a second exon, an IRES element, a coding region, a first exon, and a 5′ intron, wherein the IRES element is an EV29 IRES comprising a sequence that is at least 90% identical to SEQ ID NO:
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A circular RNA molecule comprising EV29 IRES.
Dependencies are inferred from the claim text.