Elite event canola ns-b50027-4
AssigneeNuseed Nutritional Australia Pty Ltd
InventorsMalcolm Devine, Antonio Leonforte, Nelson Gororo, Greg Buzza, Shunxue Tang, Wenxiang Gao, James Petrie, Surinder Singh
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Abstract
The present embodiments relate to elite event NS-B50027-4, seeds and oils obtained from NS-B50027-4, progeny derived from NS-B50027-4, the genetic and phenotypic characteristics of NS-B50027-4, and compositions and methods for the identification of elite event NS-B50027-4. In particular, NS-B50027-4 is a transgenic canola line capable of producing at least 5% DHA in its seed oil.
Claims12
A method of detecting the presence of event NS-B50027-4 or progeny thereof in a sample comprising plant DNA, wherein said method comprises
- (a) contacting said sample with:
- at least one primer specific for the 5′ flanking junction region between the A02 insert and the native Brassica chromosome A02 DNA, said 5′ flanking junction region ranges from nucleotides 2033 to 2132 of SEQ ID NO:40, or
- at least one primer specific for the 3′ flanking junction region between the A02 insert and the native Brassica chromosome A02 DNA, said 3′ flanking junction region ranges from nucleotides 14156 to 14255 of SEQ ID NO:40, or
- at least one primer specific for the 5′ flanking junction region between the A05 insert and the native Brassica chromosome A05 DNA, said 5′ flanking junction region ranges from nucleotides 1110 to 1209 of SEQ ID NO:41, or
- at least one primer specific for the 3′ flanking junction region between the A05 insert and the native Brassica chromosome A05 DNA, said 3′ flanking junction region ranges from nucleotides 47724 to 47823 of SEQ ID NO:41, or
- at least ten or fifteen contiguous nucleotides or complements thereof,
- (b) subjecting said sample to polymerase chain reaction;
- and
- (c) assaying for amplicons generated between said primers,
- wherein said method is, optionally, a competitive allele-specific PCR-based genotyping assay;
- and
- wherein said method is diagnostic of elite event NS-B50026-4, seed characteristic of which has been deposited under ATCC Accession No. PTA-123186.
A method of detecting the presence of event NS-B50027-4 or progeny thereof in a sample comprising plant DNA, wherein said method comprises
- (a) contacting said sample with:
- at least one primer specific for the 5′ flanking junction region between the A02 insert and the native Brassica chromosome A02 DNA, said 5′ flanking junction region ranges from nucleotides 2040 to 2139 of SEQ ID NO:40, or
- at least one primer specific for the 3′ flanking junction region between the A02 insert and the native Brassica chromosome A02 DNA, said 3′ flanking junction region ranges from nucleotides 14152 to 14251 of SEQ ID NO:40, or
- at least one primer specific for the 5′ flanking junction region between the A05 insert and the native Brassica chromosome A05 DNA, said 5′ flanking junction region ranges from nucleotides 1110 to 1209 of SEQ ID NO:41, or
- at least one primer specific for the 3′ flanking junction region between the A05 insert and the native Brassica chromosome A05 DNA, said 3′ flanking junction region ranges from nucleotides 47724 to 47823 of SEQ ID NO:41, or
- at least ten or fifteen contiguous nucleotides or complements thereof,
- (b) subjecting said sample to polymerase chain reaction;
- and
- (c) assaying for amplicons generated between said primers,
- wherein said method is, optionally, a competitive allele-specific PCR-based genotyping assay;
- and
- wherein said method is diagnostic of elite event NS-B50026-4, seed characteristic of which has been deposited under ATCC Accession No. PTA-123186.
Dependencies are inferred from the claim text.