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US 2022/0177897 A1Published application
Transcriptional relay system
AssigneeOctant, Inc.
InventorsLeon Yen-Lee CHAN, Aaron Ross COOPER, Henry CHAN
Life of this patent
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Abstract
Described herein are transcriptional relay systems useful for reducing background signal in protein expression and reporter assays. These systems utilize a nucleic acid system wherein a promoter sequence controls expression of a synthetic transcription factor that activates transcription of a reporter molecule.
Claims28
A transcriptional relay system comprising;
- a) a transcription factor nucleic acid comprising a response element regulated promoter nucleotide sequence and a nucleotide sequence encoding a synthetic transcription factor, wherein said response element regulated promoter nucleotide sequence is 5′ to said nucleotide sequence encoding said synthetic transcription factor;
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- b) a reporter nucleic acid comprising a synthetic transcription factor promoter nucleotide sequence and a nucleotide sequence encoding a reporter, wherein said synthetic transcription factor promoter nucleotide sequence is 5′ to said nucleotide sequence encoding said reporter, and wherein said synthetic transcription factor promoter nucleotide sequence is able to be bound by said synthetic transcription factor.
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Dependencies are inferred from the claim text.