Recombinant microorganism including genetic modification that increases activity of nitrous oxide reductase pathway and method of reducing concentration of nitrous oxide in sample by using the same

A composition for reducing a concentration of nitrous oxide in a sample, the composition comprising:

• a recombinant microorganism of the genus Escherichia comprising a genetic modification that increases expression of a nosZ gene encoding NosZ, which is a nitrous oxide reductase, in the recombinant microorganism,
• wherein the recombinant microorganism comprises the nosZ gene, a nosR gene encoding NosR, a nosD gene encoding NosD, a nosF gene encoding NosF, a nosY gene encoding NosY, and an apbE gene encoding ApbE, and wherein the nosZ gene, the nosR gene, the nosD gene, the nosF gene, the nosY gene and the apbE gene are derived from a microorganism of the genus Pseudomonas, the genus Paracoccus, or a combination thereof.

A method of reducing a concentration of nitrous oxide in a sample, the method comprising contacting a recombinant microorganism with the nitrous oxide-containing sample to reduce the concentration of nitrous oxide in the sample,

• wherein the recombinant microorganism is of the genus Escherichia and comprises a genetic modification that increases expression of a nosZ gene encoding NosZ, which is a nitrous oxide reductase, in the recombinant microorganism,
• wherein the recombinant microorganism comprises the nosZ gene, a nosR gene encoding NosR, a nosD gene encoding NosD, a nosF gene encoding NosF, a nosY gene encoding NosY, and an apbE gene encoding ApbE, wherein the nosZ gene, the nosR gene, the nosD gene, the nosF gene, the nosY gene, and the apbE gene are derived from a microorganism of the genus Pseudomonas, the genus Paracoccus, or a combination thereof.