Back to results
US 2022/0177860 A1Published application
Pdcd-1 homing endonuclease variants
Assignee2seventy bio, Inc.
InventorsJORDAN JARJOUR, KYLE HAVENS, JASDEEP MANN
Life of this patent
Estimating the term…
Abstract
The present disclosure provides improved homing endonuclease variants and megaTALs reprogrammed to bind and cleave the PDCD-1 gene.
Claims21
1-55. (canceled)
A polypeptide comprising an I-OnuI homing endonuclease (HE) variant, wherein the polypeptide comprises an amino acid sequence at least 98% identical to the amino acid sequence set forth in SEQ ID NO:
- 6.
A polypeptide comprising an amino acid sequence at least 98% identical to the amino acid sequence set forth in SEQ ID NO:
- 7.
A method of editing a human PDCD-1 gene in a cell comprising:
- introducing a polynucleotide encoding the polypeptide of claim 1 into the cell, wherein expression of the polypeptide creates a double strand break at a target site in a human PDCD-1 gene.
Dependencies are inferred from the claim text.