Polymerase enzyme from phage t4
AssigneeIsoPiexis Corporation
InventorsJerzy Olejnik, Angela Delucia, Ralf Peist, Nicole Grasse
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Abstract
The present invention relates to a polymerase enzyme with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising the following mutations in the motif A region (SGS). It relates to a polymerase enzyme according to SEQ ID NO. 1 or any polymerase that shares at least 70% amino acid sequence identity thereto, comprising a mutation selected from the group of (i) at position 412 of SEQ ID NO. 1: serine (S) (L412S) and/or, (ii) at position 413 of SEQ ID NO. 1: glycine (G) (Y413G) and/or (iii) at position 414 of SEQ ID NO. 1: serine (S) (P414S), wherein the enzyme has little or no 3′-5′ exonuclease activity.
Claims15
A polymerase enzyme according to SEQ ID NO. 1 or any polymerase that shares at least 70%, 80%, 90%, 95% or, 98% amino acid sequence identity thereto, comprising the following mutation(s):
- i. at position 412 of SEQ ID NO. 1:
- serine (S), glutamine (Q), tyrosine (Y) or phenylalanine (F) and/or (L412S, L412Q, L412Y, L412F)
- ii. at position 413 of SEQ ID NO. 1:
- glycine (G), alanine (A), serine (S) and/or (Y413G, Y413A, Y413S),
- iii. at position 414 of SEQ ID NO. 1:
- serine (S), valine (V), isoleucine (I), cysteine (C), alanine (A) (P414S, P414I, P414V, P414C, P414A)
- wherein the enzyme has little or no 3′-5′ exonuclease activity.
Dependencies are inferred from the claim text.