Loading patent…
AssigneeProvivi, Inc.
InventorsEffendi LEONARD, Micah SHEPPARD, Thomas HEEL, Kathryn Christina WU
Estimating the term…
The present application relates to methods to improve biomass or lipid production in a microorganism from one or more fatty acid and one or more simple carbon co-substrates. Produced lipids may include unsaturated C6-C24 fatty acids, alcohols, aldehydes, and acetates which may be useful as final products or precursors to insect pheromones, fragrances, flavors, and polymer intermediates. The application further relates to recombinant microorganisms modified for improved production of biomass or lipid, or improved lipid selectivity. Also provided are methods of producing one or more lipid using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally one or more of the product lipid.
A method for increasing the selectivity of a Z11-16 non-native unsaturated fatty acid in a recombinant Yarrowia lipolytica microorganism, said method comprising the steps of:
A recombinant Yarrowia lipolytica microorganism with improved Z11-16 acid selectivity, said recombinant Yarrowia lipolytica microorganism comprising a heterologous decarboxylating malic enzyme from an organism selected from the group consisting of Escherichia coli, Hominidae sapiens, Lypomyces starkeyi, Mus musculus, Rattus novegicus, and Rhodosporidium toruloides, wherein the Z11-16 acid selectivity is increased compared to a control microorganism without the nucleic acid sequence encoding the decarboxylating malic enzyme.
Dependencies are inferred from the claim text.