Adeno-associated viral vector producer cell lines
AssigneeGlaxoSmithKline Intellectual Property Development Limited
InventorsSimon Alexander CHANAS, Conrad VINK
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Abstract
The invention relates to an adeno-associated virus (AAV) vector producer cell comprising nucleic acid sequences encoding AAV rep and cap genes, helper virus genes, and a DNA genome of the AAV vector; the AAV rep gene comprising an intron, the intron comprising a transcription termination 5 sequence with a first recombination site located upstream and a second recombination site located downstream of the transcription termination sequence; and the nucleic acid sequences all integrated together at a single locus within the AAV vector producer cell genome. The invention also relates to methods for producing the AAV vector producer cell lines.
Claims30
An adeno-associated virus (AAV) vector producer cell comprising nucleic acid sequences encoding:
- AAV rep and cap genes,
- helper virus genes, and
- a DNA genome of the AAV vector;
- wherein the AAV rep gene comprises an intron, said intron comprising a transcription termination sequence with a first recombination site located upstream and a second recombination site located downstream of said transcription termination sequence;
- and
- wherein said nucleic acid sequences are all integrated together at a single locus within the AAV vector producer cell genome.
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A nucleic acid vector comprising a non-mammalian origin of replication and the ability to hold at least 25 kilobases (kb) of DNA, characterized in that said nucleic acid vector comprises nucleic acid sequences encoding:
- AAV rep and cap genes;
- helper virus genes;
- and
- a DNA genome of an AAV vector;
- wherein the rep gene comprises an intron, said intron comprising a transcription termination sequence with a first recombination site located upstream and a second recombination site located downstream of the transcription termination sequence;
- and
- wherein the nucleic acid sequences encoding the AAV rep and cap genes, each of the helper virus genes and the DNA genome of the AAV vector are arranged as individual expression cassettes within the nucleic acid vector.
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Dependencies are inferred from the claim text.