Additive composition for nk cell culture medium, culture method for nk cell by using same additive composition, and cosmetics composition obtained thereby for improving skin problems

2:

• The additive composition of claim 1, wherein the active ingredient is a steroid and functions to increase the proliferation rate of NK cells.

3:

• The additive composition of claim 2, wherein the steroid is glucocorticoids.

4:

• The additive composition of claim 3, wherein the glucocorticoid is one or more substances selected from the group consisting of cortisol, cortisone, prednisolone, prednisone, methylprednisolone, dexamethasone, betamethasone, triamcinolone, fludrocortisone acetate, and deoxycorticosterone acetate.

(canceled)

6:

• The additive composition of claim 2, wherein the steroid is contained at a concentration of 0.2 μm/ml or higher.

8:

• The method of claim 7, wherein the NK cell stimulation substance is at least one selected from the group consisting of an anti-CD16 antibody, an anti-CD56 antibody, and IL-2, IL-12, IL-18;
• and
• the T cell stimulation substance is at least one selected from the group consisting of an anti-CD3 antibody, an anti-CD4 antibody, and an anti-CD28 antibody.

9:

• The method of claim 7, wherein the Treg activity suppressing substance is a steroid;
• and
• the steroid inhibits the immunosuppressive activity of regulatory T cells (Tregs), thereby helping the proliferation of the NK cells.

10:

• The method of claim 7, wherein the treating of the culture medium with the T cell stimulation substance is performed on the second day of culture;
• and
• the treating of the culture medium with the additive composition is performed on the third day of culture.

11:

• The method of claim 10, wherein the treating of the culture medium with the additive composition is performed again one or more times on or after the fifth day of culture.

12:

• The method of claim 7, wherein the additive composition comprises a steroid at a concentration of 0.2 ug/mL or more;
• and
• the steroid is dissolved in a carrier or cytokine 1-1 solution, wherein the cytokine 1-1 solution is obtained by dissolving IL-12 and IL-18 in a basic solution such that the IL-12 and the IL-18 are contained at a concentration of 0.5-5 ng/mL and a concentration of 2 of 50 ng/mL, respectively in the basic solution.

13:

• The method of claim 7, further comprising:
• harvesting the NK cells after culturing the NK cells for 11 to 16 days after the treating of the culture medium with the additive composition.

14:

• An immune cell therapeutic agent comprising NK cells obtained by the NK cell culture method of claim 13 as an active ingredient.

17:

• A composition comprising the NK cell culture medium composition obtained by the method of claim 16 as an active ingredient.

18:

• A method for treating a skin disease, the method comprising applying the composition of claim 17 to a subject with the skin disease.

19:

• The method of claim 12, further comprising:
• harvesting the NK cells after culturing the NK cells for 11 to 16 days after the treating of the culture medium with the additive composition.

20:

• An immune cell therapeutic agent comprising NK cells obtained by the NK cell culture method of claim 19 as an active ingredient.

21:

• A method for alleviating a skin trouble, the method comprising applying the composition of claim 17 to a subject with the skin trouble.