Geometric induction of pluripotency
OwnerStefania CARELLI, Manuela Teresa RAIMONDI +5inventors
InventorsStefania CARELLI, Manuela Teresa RAIMONDI, Anna Maria DI GIULIO, Toniella GIALLONGO, Alfredo GORIO, Giulio Nicola CERULLO, Roberto OSELLAME
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Abstract
A method for culturing cells on a substrate capable of inducing pluripotency is provided. The method includes plating cells on a nichoid-type substrate, allowing cultured cells to proliferate for a certain period of time, detaching cells from the nichoid-type substrate, and, once cells have been detached, culturing cells in suspension or under adhesion.
Claims21
A method for inducing pluripotency in cells by using a nichoid-type substrate.
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A method of differentiating stem cells comprising the steps of:
- i) plating said cells on a nichoid-type substrate in the presence of a culture medium which does not comprise serum,
- ii) replacing the culture medium with a medium which comprises serum, and
- iii) harvesting the differentiated cells,
- wherein step i) is carried out in the absence of a cell adhesion-promoting substrate.
(canceled)
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Dependencies are inferred from the claim text.