All-in-one bioreactor for therapeutic cells manufacturing

A method of performing a transfection process and an expansion process to a plurality of cells in a bioreactor, comprising:

• flowing a first cell culture media into an upper reservoir of the bioreactor having a plurality of cells disposed therein via a fluid input port of the upper reservoir;
• flowing a transfection media or suspension comprising a molecular species for genetically modifying the plurality of cells through a lower compartment of the bioreactor, wherein the upper reservoir and the lower compartment are separated by a membrane having a plurality of micro-straws extending therethrough;
• performing a transfection process to the plurality of cells in the upper reservoir by injecting the molecular species from the transfection media or suspension into the plurality of cells via the plurality of micro-straws to form a plurality of genetically modified cells;
• and
• expanding the plurality of genetically modified cells in the upper reservoir via the first cell culture media.

A non-transitory computer readable medium having instructions stored thereon that, when executed by a processor, causes a bioreactor to perform a method comprising:

• flowing a first cell culture media into an upper reservoir of the bioreactor having a plurality of cells disposed therein via a fluid input port of the upper reservoir;
• flowing a transfection media or suspension comprising a molecular species for genetically modifying the plurality of cells through a lower compartment of the bioreactor, wherein the upper reservoir and the lower compartment are separated by a membrane having a plurality of micro-straws extending therethrough;
• performing a transfection process to the plurality of cells in the upper reservoir by injecting the molecular species from the transfection media or suspension into the plurality of cells via the plurality of micro-straws to form a plurality of genetically modified cells;
• and
• expanding the plurality of genetically modified cells in the upper reservoir via the first cell culture media.