Microfluidic system for intracellular delivery of materials and method therefor
AssigneeMxt Biotech
InventorsAram CHUNG, Geoum-Young KANG, Jeong-Soo HUR
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Abstract
There is provided a microfluidic system delivering external materials into a cell by cell mechanoporation using inertia, the microfluidic system including a fluidic channel structure through which a solution containing a cell and external materials flows continuously, in which the fluidic channel structure includes a junction between one or more channels, a localized vortex is generated near an interface of the junction, the cell is deformed by the vortex, and transient discontinuities are generated in a cell membrane by the vortex and the external materials are introduced into the cell by solution exchange between the cell and fluid around the cell.
Claims16
A microfluidic system delivering external materials into a cell by cell mechanoporation using inertia, the microfluidic system comprising:
- a fluidic channel structure through which a solution containing a cell and external materials flows continuously,
- wherein the fluidic channel structure includes a junction between one or more channels,
- a localized vortex is generated near an interface of the junction,
- the cell is deformed by the vortex, and
- transient discontinuities are generated in a cell membrane by the vortex and the external materials are introduced into the cell by solution exchange between the cell and fluid around the cell.
A method of delivering external materials into a cell by cell mechanoporation using inertia, the method comprising:
- allowing a solution containing the cell and external materials to continuously flow a fluidic channel;
- forming a vortex by a vortex generating means near the junction;
- deforming the cell by the vortex;
- and
- allowing the external materials to be introduced into the cell through a pore created in a cell membrane by the deforming of the cell.
Dependencies are inferred from the claim text.