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US 2022/0177655 A1Published application
Method for deacetylation of biopolymers
AssigneeGalderma Holding SA
InventorsJohan Olsson, Craig Steven Harris
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Abstract
A method for at least partial deacetylation of a biopolymer comprising acetyl groups, including: a1) providing a biopolymer including acetyl groups; a2) reacting the biopolymer including acetyl groups with hydroxylamine (NH2OH) or a salt thereof at a temperature of 100° C. or less for 2-200 hours to form an at least partially deacetylated biopolymer; and a3) recovering the at least partially deacetylated biopolymer.
Claims20
A method of preparing a hydrogel product comprising crosslinked glycosaminoglycan molecules, the method comprising:
- (a) obtaining a solution comprising an at least first glycosaminoglycan that is a partially deacetylated glycosaminoglycan with a degree of acetylation between 50% and 99% and a second glycosaminoglycan, wherein the second glycosaminoglycan is different from the at least partially deacetylated glycosaminoglycan;
- (b) activating carboxyl groups on the at least partially deacetylated glycosaminoglycan and/or the second glycosaminoglycan with a coupling agent, to form activated glycosaminoglycans;
- (c) crosslinking the activated glycosaminoglycans using a crosslinker comprising at least two amine groups solely with amide bonds to provide crosslinked glycosaminoglycans;
- and
- (d) acylating residual amine groups of the crosslinked glycosaminoglycans obtained in (c) to form acylated crosslinked glycosaminoglycans;
- and
- (e) subjecting the crosslinked glycosaminoglycans provided in (c) or (d) to alkaline treatment to hydrolyze ester crosslinks formed as byproducts during the amide crosslinking in (c),
- wherein the first and second glycosaminoglycans are not the crosslinker, and wherein the crosslinker comprises a di-, tri-, or tetra-saccharide, oligosaccharide, or polysaccharide.
5 more elements
Dependencies are inferred from the claim text.