Restricted immunoglobulin heavy chain mice
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsLynn Macdonald, John McWhirter, Andrew J. Murphy
Life of this patent
Estimating the term…
Abstract
Mice having a restricted immunoglobulin heavy chain locus are provided, wherein the locus is characterized by a single polymorphic human VH gene segment, a plurality of human DH gene segments and a plurality of JH gene segments. Methods for making antibody sequences that bind an antigen (e.g., a viral antigen) are provided, comprising immunizing a mouse with an antigen of interest, wherein the mouse comprises a single human VH gene segment, a plurality of human DH gene segments and a plurality of JH gene segments, at the endogenous immunoglobulin heavy chain locus.
Claims25
A rat or mouse having a restricted immunoglobulin heavy chain locus characterized by the presence of a single human VH gene segment, one or more human DH gene segments, and one or more human JH gene segments.
-5. (canceled)
A mouse comprising, in its germline, a replacement at an endogenous immunoglobulin heavy chain locus of all or substantially all endogenous VH, DH, and JH gene segments with single human VH gene segment and/or polymorphic variants thereof, one or more human DH gene segments and one or more human JH gene segments.
(canceled)
-19. (canceled)
A rat or mouse comprising an endogenous immunoglobulin heavy chain locus, wherein the endogenous immunoglobulin heavy chain locus consists essentially of
- a) one or more polymorphic variants of a single human VH gene segment,
- b) one or more human DH gene segments, and
- c) one or more human JH gene segments,
- wherein the single human VH gene segment is a polymorphic VH gene segment associated with a high copy number in human populations, and
- wherein the single human VH gene segment, one or more human DH gene segments, and one or more JH gene segments are operably linked to a non-human immunoglobulin heavy chain constant region gene.
Dependencies are inferred from the claim text.