ANTI-TNF/IFN scFv-Fc BISPECIFIC ANTIBODY AND USES THEREOF
AssigneeJp Duetbio Co., Ltd
InventorsDong-Il KIM, Hyun-Myoung Cha, Jin-Hyuk Lim
Life of this patent
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Abstract
Disclosed is anti-TNF/IFN scFv-Fc bispecific antibody and a pharmaceutical composition for prevention or treatment of inflammatory bowel disease, the composition containing the antibody. The bispecific antibody has a structure in which an Fc fragment is conjugated to an scFv bispecific antibody. Thus, a purification process thereof may be easy. The bispecific antibody may effectively and simultaneously conjugate to TNF-α and IFN-γ which independently play an important role in inducing Crohn's disease. Thus, the bispecific antibody may exhibit excellent effects in the treatment of inflammatory bowel disease including Crohn's disease.
Claims17
A polynucleotide encoding a bispecific antibody having binding specificity to TNF-α and IFN-γ, the polynucleotide containing anti-TNF scFv-Fc represented by SEQ ID NO:
- 1 and anti-IFN scFv-Fc represented by SEQ ID NO:
- 2.
A bispecific antibody having binding specificity to TNF-α and IFN-γ, the bispecific antibody containing anti-TNF scFv-Fc represented by SEQ ID NO:
- 3 and anti-IFN scFv-Fc represented by SEQ ID NO:
- 4.
A method for preparing a bispecific antibody having binding specificity to TNF-α and IFN-γ, the method comprising:
- 1) preparing a transformant by introducing an anti-TNF scFv-Fc represented by SEQ ID NO:
- 1 and an anti-IFN scFv-Fc represented by SEQ ID NO:
- 2 into a host cell;
- and
- 2) culturing the transformant in a medium and then separating and purifying an anti-TNF/IFN scFv-Fc bispecific antibody expressed in the medium.
Dependencies are inferred from the claim text.