Antibodies to Pyroglutamate Amyloid-B and Uses Thereof

A method of detecting amyloid-β peptide having pyroglutamate at the third residue (3pE Ab) or a fragment thereof in a biological sample, the method comprising:

• (i) contacting the biological sample with an isolated monoclonal antibody or antigen binding fragment that specifically binds 3pE Aβ or a fragment thereof, and
• (ii) determining whether an immune complex is formed between the isolated monoclonal antibody or antigen binding fragment thereof and the 3pE Aβ or fragment thereof;
• wherein the isolated monoclonal antibody or antigen-binding fragment thereof comprises a heavy complementarity determining region 1 (HCDR1), HCDR2, HCDR3, and a light chain complementarity determining region 1 (LCDR1), LCDR2, and LCDR3, having the polypeptide sequences of:
• a. SEQ ID NOs:
• 1, 2, 3, 4, 5, and 6, respectively;
• b. SEQ ID NOs:
• 1, 7, 3, 4, 5, and 6, respectively;
• c. SEQ ID NOs:
• 1, 7, 3, 8, 5, and 6, respectively;
• d. SEQ ID NOs:
• 1, 2, 3, 8, 5, and 6, respectively;
• e. SEQ ID NOs:
• 56, 57, 3, 8, 5, and 6, respectively;
• f. SEQ ID NOs:
• 56, 57, 3, 4, 5, and 6, respectively;
• g. SEQ ID NOs:
• 56, 58, 3, 4, 5, and 6, respectively;
• h. SEQ ID NOs:
• 56, 7, 3, 8, 5, and 6, respectively;
• i. SEQ ID NOs:
• 1, 57, 3, 8, 5, and 6, respectively;
• j. SEQ ID NOs:
• 56, 7, 3, 4, 5, and 6, respectively;
• k. SEQ ID NOs:
• 1, 57, 3, 4, 5, and 6, respectively;
• l. SEQ ID NOs:
• 1, 58, 3, 4, 5, and 6, respectively;
• or
• m. SEQ ID NOs:
• 56, 2, 3, 4, 5, and 6, respectively.