Mhc class ii molecule-based peptide exchange system and method
AssigneeMBL International Corp.
InventorsMarc Delcommenne, Yuri Poluektov
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Abstract
The present disclosure relates to compositions, kits, and methods to perform peptide exchange on MHC class II molecules, such as quantified peptide exchange.
Claims77
A protein complex comprising an MHC class II molecule bound to a first peptide, wherein the first peptide is labeled with a first label.
The protein complex of any preceding claim, wherein the first peptide is PKPVSKMRMATPLLM, PKPVSLMRMATPLLM, KPVSKMRMARPLLMQ, PKPVSKYRMATPLLM, or a functional homologue thereof having at least 90% identity with PKPVSKMRMATPLLM, PKPVSLMRMATPLLM or PKPVSKYRMATPLLM.
The protein complex of any preceding claim, wherein the first peptide is PKPVSKMRMATPLLM, PKPVSLMRMATPLLM, KPVSKMRMARPLLMQ, or PKPVSKYRMATPLLM.
The protein complex of any preceding claim, wherein the first label is biotin, a hapten, or a tag.
The protein complex of any preceding claim, wherein the first label is a first tag.
The protein complex of any preceding claim, wherein the first tag is DNP.
The protein complex of any preceding claim, wherein the MHC class II molecule is HLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRA, and HLA-DRB1, or I-A, I-E in mice.
The protein complex of any preceding claim, wherein the protein complex can be used for quantified or non-quantified peptide exchange.
A kit for peptide exchange comprising an MHC class II molecule bound to a first peptide wherein the first peptide is labeled with a first label.
A method of performing peptide exchange on an MHC class II molecule, comprising:
- providing an MHC class II molecule bound to a first peptide, wherein the first peptide is labeled with a first label;
- contacting the MHC class II molecule bound to the first peptide with a second binding partner, wherein the contacting step generates an MHC class II molecule bound to the second binding partner.
Dependencies are inferred from the claim text.