Method for purifying protein
AssigneeAsahi Kasei Medical Co., Ltd.
InventorsAkika FUTAMURA, Hiroki TANIGUCHI, Junji HIDAKA
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Abstract
A method for purifying a protein-containing solution comprising a protein of interest, the method comprising the steps of: (a1) contacting the protein-containing solution with a porous medium comprising polyketone; and (a2) passing the protein-containing solution contacted with the porous medium comprising polyketone through a virus removal membrane.
Claims38
A method for purifying a protein-containing solution comprising a protein of interest, the method comprising:
- (a1) contacting the protein-containing solution with a porous medium comprising polyketone;
- and
- (a2) passing the protein-containing solution contacted with the porous medium comprising polyketone through a virus removal membrane.
A method for purifying a protein-containing solution comprising a protein of interest, the method comprising:
- (b1) contacting the protein-containing solution with a porous medium having a specific surface area of 4.0 m2/mL or larger and a porosity of 50% or more;
- and
- (b2) passing the protein-containing solution contacted with the porous medium through a virus removal membrane.
A prefilter for a virus removal membrane, comprising a porous medium comprising polyketone.
A method for recovering a purified protein from a protein-containing solution, the method comprising:
- (a1) contacting the protein-containing solution with a medium comprising polyketone;
- and
- (a2) recovering the purified protein from the protein-containing solution contacted with the medium comprising polyketone.
A method for selectively removing a protein aggregate contained in a protein-containing solution from the protein-containing solution, the method comprising:
- (b1) contacting the protein-containing solution with a medium comprising polyketone.
A medium for selectively removing a protein aggregate from a protein-containing solution, the medium comprising polyketone.
Use of polyketone for selectively removing a protein aggregate from a protein-containing solution.
Dependencies are inferred from the claim text.