Process for the purification of recombinant polypeptides
AssigneeGlaxoSmithKline Intellectual Property Development Limited
InventorsDaniel GIBSON, Richard KUCIA-TRAN, Nehal PATEL, Trevor WILEY
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Abstract
The present invention is directed to a novel process for purifying a recombinant polypeptide from a solution comprising one or more impurities, wherein the process is a chromatography process which uses saccharin. The present invention also provides the use of saccharin in a process for purifying a recombinant polypeptide from a solution comprising one or more impurities, wherein the process is a chromatography process. The invention further provides a wash buffer for purifying using chromatography a recombinant polypeptide from a solution comprising one or more impurities, wherein the wash buffer comprises saccharin.
Claims22
A process for purifying a recombinant polypeptide from a solution comprising one or more impurities, wherein the process is a chromatography process which comprises the addition of saccharin, wherein the saccharin concentration is from about 0.01 M to about 4 M, and wherein the saccharin is not used to displace the recombinant polypeptide from a chromatography support.
A wash or load buffer for purifying using chromatography a recombinant polypeptide from a solution comprising one or more impurities, wherein the wash or load buffer comprises saccharin, and wherein the saccharin concentration is from about 0.01 M to about 4 M.
A cell culture feedstream comprising a recombinant polypeptide and one or more impurities, wherein the feedstream is a solution comprising saccharin, and wherein the saccharin concentration is from about 0.01 M to about 4 M.
(canceled)
A process for purifying an antibody from a solution comprising the antibody and one or more impurities derived from a mammalian cell, the process comprising a chromatography process comprising a chromatography support and:
- (a) loading the solution comprising the antibody and one or more impurities derived from a mammalian cell on to the chromatography support by applying the solution and a loading buffer to the chromatography support;
- (b) washing the chromatography support by applying a washing buffer to the chromatography support;
- (c) eluting the antibody from the chromatography support by applying an elution buffer to the chromatography support;
- wherein one or more of the loading buffer and the washing buffer comprise from about 0.3 M to about 1.5 M saccharin.
Dependencies are inferred from the claim text.