Devices, cartridges, and sensors for analyzing a biological sample

A system, comprising

• (a) a cartridge configured to analyze a biological sample, the cartridge comprising:
• (i) a sensor comprising:
• a first channel segment;
• a second channel segment;
• a pore or a channel fluidly connecting the first channel segment and the second channel segment;
• an electrode pair configured to apply a multiplexed electrical current or voltage to the pore or channel;
• and
• an electrode pair configured to detect impedance at a plurality of frequencies within the pore or channel, wherein the electrode pair configured to apply the multiplexed electrical current and the electrode pair configured to detect the impedance are the same electrode pair or different electrode pairs;
• and
• (ii) a device interface electrically connected to the sensor;
• and
• (b) a device configured to interface with and operate the cartridge, comprising one or more processors and a non-transitory computer-readable storage medium storing one or more programs configured to be executed by the one or more processors, the one or more programs comprising instructions for determining a cell count based on a detected impedance, wherein the detected impedance comprises at least a first multivariate impedance pattern at a first frequency and a second multivariate impedance pattern at a second frequency.

A system, comprising

• (a) a cartridge configured to analyze a biological sample, the cartridge comprising:
• (i) a sensor comprising:
• a first channel segment;
• a second channel segment;
• a pore or a channel fluidly connecting the first channel segment and the second channel segment;
• an electrode pair configured to apply a multiplexed electrical current or voltage to the pore or channel;
• and
• an electrode pair configured to detect impedance at a plurality of frequencies within the pore or channel, wherein the electrode pair configured to apply the multiplexed electrical current and the electrode pair configured to detect the impedance are the same electrode pair or different electrode pairs;
• and
• (ii) a device interface electrically connected to the sensor;
• and
• (b) a device configured to interface with and operate the cartridge, comprising one or more processors and a non-transitory computer-readable storage medium storing one or more programs configured to be executed by the one or more processors, the one or more programs comprising instructions for differentiating between different types of cells based on the detected impedance using a machine learning model, wherein the detected impedance comprises at least a magnitude component and a phase component of impedance at a first frequency and a magnitude component and a phase component of impedance at a second frequency.