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US 2022/0176375 A1Published application
Methods and systems for microfluidic screening
Assignee1859, Inc.
InventorsDevon CAYER, Andrew MACCONNELL, Pavel CHUBUKOV, Ramesh RAMJI, Sean STROMBERG
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Abstract
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
Claims20
A method for amplifying a primer to maximize cellular nucleic acid capture, the method comprising:
- (a) providing an encapsulation encapsulating a scaffold, one or more cells, an amplification mix, and a nicking enzyme, wherein a nucleic acid encoding is bound to the scaffold;
- (b) lysing the one or more cells to release one or more cellular nucleic acids;
- (c) nicking the nucleic acid encoding with the nicking enzyme, thereby creating a first encoded nucleic acid primer;
- (d) amplifying the first encoded nucleic acid primer via the nicking site and amplification mix;
- and
- (e) labeling the one or more cellular nucleic acids with the first encoded nucleic acid primer.
3 more elements
A system for amplifying a primer to maximize cellular nucleic acid capture, the system comprising:
- (a) a scaffold, wherein a nucleic acid encoding is bound to the scaffold;
- (b) one or more cells each comprising one or more cellular nucleic acids;
- (c) an amplification mix;
- (d) a nicking enzyme;
- (e) a microfluidic device configured to:
- (i) receive the scaffold, the one or more cells, the amplification mix, and the nicking enzyme,
- (ii) encapsulate the scaffold, the one or more cells, the amplification mix, and the nicking enzyme in an encapsulation,
- (iii) lyse the one or more cells, such that the one or more cellular nucleic acids are released,
- (iv) nick the nucleic acid encoding with the nicking enzyme, thereby creating a first encoded nucleic acid primer, wherein the one or more cellular nucleic acids are labeled with the first encoded nucleic acid primer, and
- (v) amplify the first encoded nucleic acid primer.
7 more elements
Dependencies are inferred from the claim text.