Back to results
US 2022/0176374 A1Published application
Methods and systems for microfluidic screening
Assignee1859, Inc.
InventorsDevon CAYER, Andrew MACCONNELL, Pavel CHUBUKOV, Ramesh RAMJI, Sean STROMBERG
Life of this patent
Estimating the term…
Abstract
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
Claims20
A method for screening an encoded effector, the method comprising:
- (a) providing at least one cell and a scaffold in an encapsulation, wherein the scaffold comprises an encoded effector bound to the scaffold by a photocleavable linker and a nucleic acid encoding the effector;
- (b) cleaving the photocleavable linker to release the encoded effector from the scaffold;
- and
- (c) detecting a signal from the droplet, wherein the signal results from an interaction between the encoded effector and the at least one cell.
1 more element
A system for screening an encoded effector, the system comprising:
- (a) one or more cells;
- (b) a scaffold, wherein an encoded effector is bound to the scaffold by a cleavable linker, wherein a nucleic acid encoding the effector is bound to the scaffold;
- and
- (c) a microfluidic device configured to:
- (i) receive the one or more cells and scaffold;
- (ii) encapsulate the one or more cells and scaffold within an encapsulation;
- (iii) cleave the cleavable linker from the encoded effector to release a predetermined amount of the encoded effector within the encapsulation;
- (iv) incubate the encoded effector with the one or more cells for a period of time;
- (v) detect a signal from the encapsulation, wherein the signal results from an interaction between the encoded effector and one or more cells;
- and
- (vi) sort the encapsulation based on the detection of the signal.
8 more elements
A method for amplifying a primer to maximize cellular nucleic acid capture comprising:
- (a) providing an encapsulation comprising a nucleic acid encoded scaffold with one or more cells, an amplification mix, and a nicking enzyme, wherein a nucleic acid encoding is bound to the nucleic acid encoded scaffold;
- (b) lysing the one or more cells to release one or more cellular nucleic acids;
- (c) nicking the nucleic acid encoding with the nicking enzyme, thereby creating an encoded nucleic acid primer;
- (d) amplifying the encoded nucleic acid primer via the nicking site and amplification mix;
- and
- (e) labeling a released cellular nucleic acid with the encoded nucleic acid primer.
3 more elements
Dependencies are inferred from the claim text.