Process and apparatus for sequential synthesis of biological polymers
AssigneeNitto Denko Avecia, Inc.
InventorKarsten KELLER
Life of this patent
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Abstract
A method and apparatus for nucleic acid synthesis. The method employs a device including at least one deprotection unit to carry out a step of deprotection, at least one coupling unit to carry out a step of coupling, at least one oxidation/thiolation unit to carry out a step of oxidation orthiolation, at least one capping unit to carry out a step of capping, and at least one washing unit to carry out a step of washing. A plurality of reaction vessels for nucleic acid synthesis are moved to the units in accord with a synthesis scheme for a desired nucleic acid sequence and at least two reaction vessels are simultaneously acted upon at several of the units in series.
Claims63
A method of nucleic acid synthesis using a device comprising at least one deprotection unit to carry out a step of deprotection, at least one coupling unit to carry out a step of coupling, at least one oxidation/thiolation unit to carry out a step of oxidation or thiolation, and at least one washing unit to carry out a step of washing, wherein, a plurality of reaction vessels for nucleic acid synthesis are moved to the units in accord with a synthesis scheme for a desired nucleic acid sequence, and wherein at least two reaction vessels are simultaneously acted upon at several of the units in series.
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An apparatus for the multi-stage synthesis of organic molecules, said apparatus comprising in combination:
- (a) a plurality of reaction vessels;
- (b) a plurality of fluid reservoirs;
- (c) valve elements associated with said fluid reservoirs;
- (d) fluid delivery devices for providing a feed stream from the fluid reservoirs to said vessels;
- (e) a plurality of devices capable of monitoring a chemical composition of effluent from a plurality of said vessels;
- and (f) a conveyor having a programmed pattern suitable for transporting the vessels from fluid engagement with one fluid reservoir to a subsequent fluid reservoir sequentially according to the programmed pattern.
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An apparatus for nucleic acid synthesis, said apparatus comprising at least one detritylation station to carry out a step of deprotection;
- at least one coupling station to carry out a step of coupling;
- at least one oxidation/thiolation station to carry out a step of oxidation or thiolation;
- at least one capping station to carry out a step of capping, and at least one washing station to carry out a step of washing;
- each station including a suitable fluid reservoir;
- wherein, a plurality of nucleic acid synthesis vessels are moved to the stations via a conveyor device in accord with a synthesis scheme for building a selected nucleic acid sequence of at least 6 Mers;
- and wherein the stations are operated in parallel.
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Dependencies are inferred from the claim text.