Packing material for size exclusion chromatography and method for producing the same

A method for producing a packing material for size exclusion chromatography, comprising the following steps (1) to (3):

• (1) a first step of polymerizing monomers comprising at least glycerol 1,3-dimethacrylate and glycidyl methacrylate with a diluent being added using a radical polymerization initiator in an aqueous medium to provide porous particles containing glycidyl groups;
• (2) a second step of dispersing the porous particles containing glycidyl groups obtained in the first step into an aqueous solution containing a dissolved sugar alcohol, adding an aqueous solution containing an alkali metal hydroxide, and heating and stirring the resulting mixture to bond the sugar alcohol to the glycidyl groups;
• and
• (3) a third step of dispersing the porous particles with the sugar alcohol bonded obtained in the second step into an aqueous solution containing a mineral acid and heating and stirring the resulting dispersion to open the rings of remaining glycidyl groups.