Improved Bone Implant Matrix Comprising Proline-Rich Peptide And Method Of Preparing The Same
AssigneeIndustrie Biomediche Insubri S.A.
InventorsGiuseppe Perale, Håvard Jostein Haugen, Ståle Petter Lyngstadaas, Felice Betge
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Abstract
The present invention deals with a bone implant matrix comprising a base matrix selected from the group comprising: —acellularized or acellularized non-demineralised bone matrix of any source, —matrix of natural mineral sources, —synthetic bioceramics matrix, or combinations of the above, wherein the surface of said base matrix is coated with an statistically homo-geneous composition which is a reinforcing mixture containing at least a bio-degradable polyester or co-polymer thereof, at least a gelatine or hydrolysed gelatine and at least an artificial Proline-Rich Peptide.
Claims26
A bone implant matrix comprising:
- a base matrix the surface thereof is coated with a statistically homogeneous composition which is a reinforcing mixture containing at least a soluble polymer, at least a substance, which is able to promote the cell-rooting and the cell growth, by stimulating cell proliferation and tissue integration (“friendliness to cell”) and at least one artificial Proline-Rich Peptide wherein the base matrix is selected from the group comprising:
- acellularized or acellularized non-demineralised bone matrix of any source,
- matrix of natural mineral sources,
- synthetic bioceramics matrix,
- or mixtures thereof,
- the soluble polymer of the reinforcing mixture is a biodegradable polyester or co-polymer thereof, the “friendliness to cell” is selected from the group consisting of gelatine, hydrolysed gelatine and the artificial Proline-Rich Peptide is selected from:
- an artificial peptide comprising the amino acid sequence of Pro-X-X-Pro-Y-Y-Y-Pro-X-X-Pro-Y-Y-Pro-X-X-Pro-X-Pro-Y-Y-Y-Y-Y-Y-Pro-Y-Y-Y-Y-Y-Y-Pro-X-X-Pro-X-Pro-Y-Y-Y-Pro-Y-Y-Pro-Y-Pro-X-X-Pro-Y-Pro-Y-Y-Pro-X-X-Pro-Y-Y-Pro-X-X-Pro-Y-Y-Pro-X-X-Pro- Y-Pro-Pro-X-Pro-Pro-X-X-X-X-X-X-X-X-Pro-X-X-Pro-X-X-X-X (SEQ ID NO 1), wherein:
- a) Pro is proline;
- b) X is an amino acid independently selected from the group consisting of Ala, Ile, Leu, Met, Phe, Trp and Val, preferably Ile, Leu, Val and Met;
- c) Y, is an amino acid independently selected from the group consisting of Asn, Cys, Gln, Ser, Thr and Tyr, preferably Ser and Gln.
- or
- an artificial peptide comprising the amino acid sequence of Pro-X-X-Pro-Y-Y-Pro-X-X-Pro-Y-Y-Pro-X-X-Pro-Y-Y-Pro-Y-Pro-Pro-X-Pro-Pro (SEQ ID NO 2), wherein:
- a) Pro is proline;
- b) X is an amino acid independently selected from the group consisting of Ala, Ile, Leu, Met, Phe, Trp and Val;
- c) Y is an amino acid independently selected from the group consisting of Asn, Cys, Gln, Ser, Thr and Tyr.
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A method for preparing a bone implant matrix comprising the steps of:
- a) preparing a solution of a reinforcing mixture containing at least a soluble polymer, at least a substance, which is able to promote cell-rooting and cell growth, by stimulating cell proliferation and tissue integration and at least an artificial Proline-Rich Peptide,
- b) immersing a base matrix into said reinforcing mixture solution, and
- c) drying and degassing the matrix for removing possible solvent residues.
A method for the in vivo induction of bone, cartilage, cementum and/or dental tissue formation comprising the administration of a bone implant matrix to a subject in need thereof.
Dependencies are inferred from the claim text.