Monovalent vaccine formulation and a method for preparation thereof
AssigneeAimst University
InventorsGuruswamy PRABHAKARAN, Manickam RAVICHANDRAN, Kurunathan SINNIAH, Yean Yean CHAN
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Abstract
The present invention discloses a vaccine formulation in accordance with an illustrative embodiment. The formulation including a live attenuated cholera vaccine strain VCUSM14P; a vaccine medium having starch, cellulose, dextrose, and yeast extract; and a phosphate buffer saline.
Claims22
A monovalent vaccine formulation against Vibrio cholerae O139 comprising:
- a live attenuated cholera vaccine strain;
- a vaccine medium having starch, cellulose, dextrose, and yeast extract;
- and
- a phosphate buffer saline.
Use of a monovalent vaccine formulation comprising a live attenuated cholera vaccine strain grown in a vaccine medium having starch, cellulose, dextrose, and yeast extract in a phosphate buffer saline for treating WT V. cholerae O139.
The use of claim 12, wherein the strain is VCUSM14P.
Use of a monovalent vaccine formulation comprising a live attenuated chlorine vaccine grown in a vaccine medium having starch (3.25-6.75%), cellulose (2.50-4%), dextrose (18.25-24.50%), yeast extract (0.01-0.05%), and NaCl (0.05-0.15%) in a 1000 mL of phosphate buffer saline at pH 7.2±0.2 for treating WT V. cholerae O139.
The use of claim 14, wherein the strain is VCUSM14P.
A method for preparing a monovalent cholera vaccine against O139, the method comprising:
- culturing of live attenuated bacterial cells of strain in a vaccine growth medium;
- recovering the bacterial cells from the culture;
- and
- suspending the recovered bacterial cells in phosphate buffer saline.
Dependencies are inferred from the claim text.