Pyocyanine Demethylases and related Phenazine Degrading Agents Compositions, Methods and Systems for Interfering with Viability of Bacteria

An engineered pyocyanine demethylase or a derivative thereof, the pyocyanine demethylase having sequence

• (SEQ ID NO:
• 1)
• M1T2G3K4T5K6P7A8I9I10G11G12V13A14I15T16A17L18
• A19A20A21G22L23G24V25W26L27F28T29D30G31R32G33
• G34R35S36T37T38E39P40V41T42M43T44L45D46V47K48
• N49D50Q51V52A53K54H55D56F57G58K59P60G61M62D63
• V64G65D66M67D68I69F70S71D72I73L74S75V76D77G78
• K79Q80V81G82Y83D84G85G86A87C88F89F90T91N92V93
• T94P95D96N97P98M99T100Y101C102E103L104T105I106
• H107L108D109A110G111E112I113F114A115R116S117
• L118T119P120H121T122L123A124P125F126T127M128
• A129I130T131G132G133T134G135E136Y137A138N139
• S140K141G142E143L144T145V146S147G148V149A150
• T151P152D153E154K155Y156E157L158K159L160
• T161K162,
• modified to include at least two replacements in SEQ ID NO:
• 1 selected from
• A53 with L, N, or V
• I73 with I, E, K, L, Q, R, T or V
• A87 with C, I, T or V
• T91 with V
• M99 with M, C, F, I, K, R, V or Y
• A129 with A, C, S, T, or V
• K141 with K, S or T
• and configured to be capable of the engineered pyocyanine demethylase or a derivative thereof to demethylate pyocyanine and/or a pyocyanin-like phenazines of formula (III)
• where R1-R10 are independently selected from hydrogen, hydroxy, alkoxy, alkyl, alkenyl, alkynyl, aryl, heteroaryl, acyl, and other groups identifiable to the skilled person, and one of R1-R10 is a negatively charged substituent (formal charge of −1) such as