Methods and compositions for treating conditions using recombinant self-complementary adeno-associated virus
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsJeffrey S. Bartlett, Steven C. Ghivizzani
Life of this patent
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Abstract
Methods and compositions for treating symptoms of conditions such as but not limited to osteoarthritis and rheumatoid arthritis. The methods may feature direct intraarticular injection of a recombinant self-complementary adeno-associated virus (sc-rAAV) with a vector adapted to express a modified IL-1Ra peptide. The methods of the present invention may express a therapeutically effective amount of the modified IL-1Ra peptide so as to ameliorating symptoms associated with the condition being treated.
Claims24
1-67. (canceled)
A method comprising administering to a human subject into a location of interest a composition comprising a recombinant adeno-associated virus (rAAV) comprising a capsid and a nucleic acid vector comprising a modified IL-1Ra gene comprising a nucleic acid sequence that is at least 90% identical to SEQ ID NO:
- 2.
(canceled)
A method of delivering an IL-1Ra peptide to a chondrocyte or synoviocyte, comprising contacting the chondrocyte or synoviocyte with a composition comprising a recombinant adeno-associated virus (rAAV) comprising a capsid and a nucleic acid vector comprising a modified IL-1Ra gene comprising a nucleic acid sequence that is at least 90% identical to SEQ ID NO:
- 2.
82-85. (canceled)
A method of providing interleukin-1 receptor agonist (IL-1Ra) peptide to an area of inflammation in a subject, said method comprising:
- introducing into a location of inflammation a composition comprising a recombinant self-complementary adeno-associated virus (sc-rAAV), wherein said sc-rAAV comprises:
- a. an engineered AAV capsid;
- and
- b. a vector packaged within the capsid, said vector comprising a modified IL-1 Ra gene operably linked to a promoter, the modified IL-1 Ra gene is at least 95% identical to SEQ ID NO:
- 2 or the IL-1Ra gene encodes an IL-1Ra protein as set forth in SEQ ID NO:
- 6;
- wherein the sc-rAAV transduces the vector into cells in the location of inflammation, wherein the modified IL-1 Ra gene is expressed so as to provide the cells in the location of inflammation a therapeutically effective amount of IL-1 Ra peptide effective for reducing inflammation.
93-96. (canceled)
Dependencies are inferred from the claim text.