Pairing probiotics and prebiotics, methods for growth and use, separately and in combination

A method for producing a probiotic product designed to promote and maintain metabolic processes, comprising:

• selecting at least one probiotic organism from the group consisting of Bacillus clausii, Lactobacillus casei, Akkermansia muciniphila, Eubacterium rectale, Lactococcus lactis, Lactobacillus bulgaricus, Lactobacillus buchneri, Pediococcus acidilactici, Bifidobacterium longum, Lactobacillus brevis, Lactobacillus delbrueckii subsp. bulgaricus, Propionibacterium shermanii, Butyricicoccus pullicaecorum, Lactobacillus acidophilus, Lactobacillus rhamnosus GG, Lactobacillus gasseri, Anaerobutyricum hallii, Bifidobacterium catenulatum, and Bifidobacterium pseudocatenulatum;
• selecting a growth broth for growing the probiotic organism, wherein the growth broth comprises 500-5000 mg of Bael fiber, 500-5000 mg of Fenugreek fiber, 500-5000 mg of organic quinoa sprout, 500-5000 mg of organic chia seed, 500-5000 mg of organic millet sprout, 500-5000 mg of organic buckwheat sprout, 500-5000 mg of N-acetyl glucosamine amino glycan, 50-500 mg of chondroitin, 500-5000 mg of mucin, 500-5000 mg of Chitosan oligo, 500-5000 mg of carrot fiber, 500-5000 mg of Promitor™ corn fiber, 500-5000 mg of Acemannan™, 500-5000 mg of pea fiber, 50-1000 mg of Acacia catechu (heartwood and bark, powder and extract), 50-1000 mg of Pullulan, 50-1000 mg of Althaea officinalis (marshmallow root), and 500-5000 mg of camels milk;
• inoculating the growth broth with the probiotic organism;
• growing the probiotic organism in the growth broth;
• harvesting the probiotic organism from the growth broth;
• selecting an agar spread plate media for growing the probiotic organism, wherein the agar spread plate media comprises 500-5000 mg of Bael fiber, 500-5000 mg of Fenugreek fiber, 500-5000 mg of organic quinoa sprout, 500-5000 mg of organic chia seed, 500-5000 mg of organic millet sprout, 500-5000 mg of organic buckwheat sprout, 500-5000 mg of N-acetyl glucosamine amino glycan, 50-500 mg of chondroitin, 500-5000 mg of mucin, 500-5000 mg of Chitosan oligo, 500-5000 mg of carrot fiber, 500-5000 mg of Promitor™ corn fiber, 500-5000 mg of Acemannan™, 500-5000 mg of pea fiber, 50-1000 mg of Acacia catechu (heartwood and bark, powder and extract), 50-1000 mg of Pullulan, 50-1000 mg of Althaea officinalis (marshmallow root), and 500-5000 mg of camels milk;
• inoculating the agar spread plate with the probiotic organism harvested from the growth broth;
• growing the probiotic organism on the agar spread plate;
• harvesting the probiotic organism from the agar spread plate;
• and
• freeze-drying the probiotic organism by use of a lyophilization reagent.

A method for growing a probiotic organism, comprising:

• selecting at least one probiotic organism from the group consisting of Bacillus clausii, Lactobacillus casei, Akkermansia muciniphila, Eubacterium rectale, Lactococcus lactis, Lactobacillus bulgaricus, Lactobacillus buchneri, Pediococcus acidilactici, Bifidobacterium longum, Lactobacillus brevis, Lactobacillus delbrueckii subsp. bulgaricus, Propionibacterium shermanii, Butyricicoccus pullicaecorum, Lactobacillus acidophilus, Lactobacillus rhamnosus GG, Lactobacillus gasseri, Anaerobutyricum hallii, Bifidobacterium catenulatum, and Bifidobacterium pseudocatenulatum;
• selecting a growth broth for growing the probiotic organism, wherein the growth broth comprises 500-5000 mg of Bael fiber, 500-5000 mg of Fenugreek fiber, 500-5000 mg of organic quinoa sprout, 500-5000 mg of organic chia seed, 500-5000 mg of organic millet sprout, 500-5000 mg of organic buckwheat sprout, 500-5000 mg of N-acetyl glucosamine amino glycan, 50-500 mg of chondroitin, 500-5000 mg of mucin, 500-5000 mg of Chitosan oligo, 500-5000 mg of carrot fiber, 500-5000 mg of Promitor™ corn fiber, 500-5000 mg of Acemannan™, 500-5000 mg of pea fiber, 50-1000 mg of Acacia catechu (heartwood and bark, powder and extract), 50-1000 mg of Pullulan, 50-1000 mg of Althaea officinalis (marshmallow root), and 500-5000 mg of camels milk;
• inoculating the growth broth with the probiotic organism;
• growing the probiotic organism in the growth broth;
• harvesting the probiotic organism from the growth broth;
• selecting an agar spread plate media for growing the probiotic organism, wherein the agar spread plate media comprises 500-5000 mg of Bael fiber, 500-5000 mg of Fenugreek fiber, 500-5000 mg of organic quinoa sprout, 500-5000 mg of organic chia seed, 500-5000 mg of organic millet sprout, 500-5000 mg of organic buckwheat sprout, 500-5000 mg of N-acetyl glucosamine amino glycan, 50-500 mg of chondroitin, 500-5000 mg of mucin, 500-5000 mg of Chitosan oligo, 500-5000 mg of carrot fiber, 500-5000 mg of Promitor™ corn fiber, 500-5000 mg of Acemannan™, 500-5000 mg of pea fiber, 50-1000 mg of Acacia catechu (heartwood and bark, powder and extract), 50-1000 mg of Pullulan, 50-1000 mg of Althaea officinalis (marshmallow root), and 500-5000 mg of camels milk;
• inoculating the agar spread plate with the probiotic organism harvested from the growth broth;
• growing the probiotic organism on the agar spread plate;
• harvesting the probiotic organism from the agar spread plate;
• and
• freeze-drying the probiotic organism by use of a lyophilization reagent, wherein the lyophilization reagent includes at least two compounds selected from the group consisting of:
• glucosamine, glutamine, sucrose, mannitol, trehalose, glycerol, inositol, raffinose, inulin, powdered skim milk, activated charcoal, soluble starch, collagen powder, chondroitin sulfate, glucosamine sulfate, guar, acacia, silica, and fructooligosaccharide P95.