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US 2022/0175840 A1Published application
Universal car-t cell and preparation method and use thereof
AssigneeShanghai Longyao Biotechnology Inc., Ltd.
InventorsXuanming YANG, Yangxin FU, Xin WANG, Shengqin YE, Xiaoqing ZHANG
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Abstract
Disclosed are a universal CAR-T cell knocking out one or more of CD3 delta, CD3 gamma, CD3 epsilon and CD3 zeta, and simultaneously introducing the HSV-TK gene. Also disclosed are a method for preparing the above-mentioned CAR-T cell, a preparation comprising the CAR-T cell, and the use of the CAR-T cell.
Claims20
A universal CAR-T cell in which one or more of CD3Delta, CD3Gamma, CD3 Epsilon and CD3 zeta is knocked out.
A method of preparing a universal CAR-T cell, including the following steps:
- one or more of CD3Delta, CD3Gamma, CD3 Epsilon and CD3 zeta is knocked out in the CAR-T cell by administering the KO CD3Delta sgRNA, KO CD3Gamma sgRNA, KO CD3 Epsilon sgRNA or KO CD3 zeta sgRNA and Cas9 protein to CAR-T cell, Wherein said KO CD3Delta sgRNA comprises the nucleotide sequence set forth in any one of SEQ ID NO:1-2, said KO CD3Gamma sgRNA comprises the nucleotide sequence set forth in any one of SEQ ID NO:
- 3-5, said KO CD3 Epsilon sgRNA comprises the nucleotide sequence set forth in any one of SEQ ID NO:
- 6-7, said KO CD3 zeta sgRNA comprises the nucleotide sequence set forth in any one of SEQ ID NO:
- 8-10.
1 more element
Dependencies are inferred from the claim text.