Phytocomplex and extract of a meristematic cell line selected from a plant belonging to the genus rosa
AssigneeAethera Biotech S.R.L.
InventorsElena Sgaravatti, Giovanna Pressi, Flavia Guzzo
Life of this patent
Estimating the term…
Abstract
The present invention relates to a meristematic cell line selected from tissue, preferably callus tissue, of a plant belonging to the genus Rosa. The invention also relates to a derivative of the cell line, i.e. a phytocomplex or an extract of the cell line. The meristematic cell line is characterized by a high polysaccharide content. Furthermore, the present invention relates to the cosmetic, nutraceutical and medical use of the selected meristematic cell line or of a derivative thereof.
Claims27
A meristematic cell line derived from a plant belonging to the genus Rosa, wherein said cell line is obtained by means of a process comprising the steps of:
- 1) plating a tissue obtained from a plant of the genus Rosa onto a solid culture medium;
- 2) isolating a plurality of cellular clones;
- 3) inoculating each of the isolated clones into a liquid culture medium;
- 4) determining the polysaccharide content for each clone;
- 5) selecting the cellular clone with the highest polysaccharide content,
- wherein the solid and liquid culture media comprise salts suitable for the growth of plant cells, sucrose, naphthylacetic acid (NAA) and 6-benzyl amino purine (BAP) and
- wherein the meristematic cell line comprises an amount of polysaccharides greater than 25% w/w.
(canceled)
(canceled)
(canceled)
A process for preparing and selecting plant meristematic cells derived from a plant belonging to the genus Rosa, said cells having a polysaccharide content greater than 25% w/w relative to the dry mass of the cell line, said process comprising the steps of:
- 1) plating a tissue obtained from a plant of the genus Rosa, preferably of the species Rosa canina or the species Rosa chinensis, onto a solid culture medium;
- 2) isolating a plurality of cellular clones;
- 3) inoculating each of the isolated clones into a liquid culture medium;
- 4) determining the polysaccharide content for each clone;
- 5) selecting the cellular clone with the highest polysaccharide content,
- wherein the solid and liquid culture media comprise salts suitable for the growth of plant cells, sucrose, naphthylacetic acid (NAA) and 6-benzyl-amino purine (BAP).
(canceled)
(canceled)
Dependencies are inferred from the claim text.