Artificial Propagation Method Of Sinocyclocheilus Rhinocerous

An artificial propagation method of Sinocyclocheilus rhinocerous, comprising the following steps:

• placing female and male parent fish in a sterilized culture container for parent fish culture, wherein black cave simulant is placed in the culture container in advance;
• the environmental parameters during parent fish culture are as follows:
• water temperature is in a range of 16-20° C., water flow velocity is in a range of 5-20 cm/s, the concentration of dissolved oxygen of water is in a range of 6.0-7.0 mg/L, and water pH is in a range of 6.8-7.2, keeping the water clean;
• keeping the culture environment dark and noiseless;
• during the parent fish culture, feeding pellet feed twice a day, and the feeding amount is according to the feed coefficient of 3%-4%, the pellet feed is a mixture of mash feed containing 40%-50% crude protein, earthworm, shrimp, vitamin D3 calcium tablets, multi-vitamin tablets and water, with a mass ratio of 250-280:80-100:10-20:10-20:8-20:300;
• collecting sperm and eggs for artificial inseminating after the gonads of female and male parent fish mature, rinsing the sperm-egg mixture with normal saline with osmotic pressure of 280-320 mOsm/L and temperature of 18-20° C. for 3-4 times, then splashing the sperm-egg mixture on the sterilized palmsheet, and incubating the palmsheet adhered with fertilized eggs in the incubating pond for 7-8 days, breeding the incubated fries to obtain the adult fish of Sinocyclocheilus rhinocerous.