In-Vitro Photoautotrophic Propagation of Cannabis
AssigneeNode Labs Inc.
InventorChristopher Leavitt
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Abstract
A plant propagation system, process and method are provided for promoting the growth of plant tissue into propagules using a photoautotrophic gel system. The plant propagation system includes a sterile growth vessel that has a vented lid to permit passive diffusion of gases. The process is initiated with one or more sterile rooted explants, which are then cultured in a large container with a vented lid photoautotrophically, which simulates ex-vitro growth conditions. These nodal explants can then be rooted onto photoautotrophic rooting agar gel in vented lid containers and subsequently transferred onto a substrate of choice for mature growth ex-vitro.
Claims29
A method of in vitro propagation of plant tissue, said method comprising:
- a. placing a first node segment of a plant in a low sugar gel media contained in a semi permeable vessel,
- b. exposing the vessel to a light environment,
- c. permitting passive diffusion of gases from the vessel for a time sufficient to facilitate photosynthetic growth of the first node segment, said photosynthetic growth resulting in the propagation of the first node segment into a propagule comprising one or more node segments.
A lid for attachment to a growth vessel having an open top, wherein said growth vessel is suitable for photoautrophic plant growth and said lid configured to seal said open top of said growth vessel, wherein said lid comprises an aperture covered by a membrane capable of permitting free exchange of gases and water vapor into and out of the growth vessel, and wherein said membrane prevents the entry of microbial pathogens into said growth vessel, wherein the size of aperture ranges from 2.5 cm to 7 cm.
Dependencies are inferred from the claim text.