Increasing plant transformability by cytotype transfer
AssigneeSyngenta Crop Protection AG
InventorsDavid Stewart SKIBBE, Sivamani ELUMALAI
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Abstract
A method of altering or transferring the cytotype of a plant line. In particular, transferring the cytotype of a transformation-recalcitrant plant line, e.g., a transformation-recalcitrant maize line, from a transformation-recalcitrant cytotype to a transformable cytotype so that the line becomes transformable while preserving its nuclear genome. The newly-transformable line may be produced using methods including backcrossing and/or haploid induction.
Claims22
A method of increasing transformation efficiency rate in a recalcitrant maize line, comprising:
- a. obtaining a recalcitrant maize plant and collecting pollen therefrom;
- b. pollinating a recipient maize plant comprising normal A (“NA”) cytoplasm with pollen from the recalcitrant maize plant;
- and
- c. obtaining a progeny embryo therefrom;
- wherein the progeny embryo comprises the NA cytoplasm and at least the nuclear genome of the recalcitrant maize plant and wherein the progeny embryo possesses a higher transformation efficiency rate than the recalcitrant maize plant.
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A method of increasing transformation efficiency rate in a recalcitrant plant line, comprising:
- a. obtaining a recalcitrant plant and collecting pollen therefrom;
- b. pollinating a recipient plant comprising transformable cytoplasm with pollen from the recalcitrant plant;
- c. obtaining a progeny embryo therefrom;
- and
- d. optionally
- i. growing the progeny tissue into a progeny plant;
- ii. backcrossing the progeny plant using the pollen from the recalcitrant plant for at least one crossing;
- and
- e. transforming tissue derived from the progeny embryo;
- wherein the progeny tissue comprises the NA cytoplasm and at least the nuclear genome of the recalcitrant plant and wherein the progeny tissue possesses a higher transformation efficiency rate than the recalcitrant plant.
A method of transforming a plant, comprising:
- a. obtaining a plurality of plant lines;
- b. testing for markers indicative of NA cytoplasm;
- c. selecting at least one line from the plurality of plant lines wherein the selected lines possess markers for NA cytoplasm;
- and
- d. transforming cells derived from the at least one selected line of step (c).
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Dependencies are inferred from the claim text.