US 2022/0171802 A1 · TIME-BASED CLUSTER IMAGING

Abstract

In an example method, a series of time-based clustering images is generated for a plurality of library fragments from a genome sample. Each time-based clustering image in the series is sequentially generated. To generate each time-based clustering image in the series: i) a respective sample is introduced to a flow cell, the respective sample including contiguity preserved library fragments of the plurality of library fragments, wherein the contiguity preserved library fragments are attached to a solid support or are attached to each other; ii) the contiguity preserved library fragments are released from the solid support or from each other; iii) the contiguity preserved library fragments are amplified to generate a plurality of respective template strands; iv) the respective template strands are stained; and v) the respective template strands are imaged.

Claims27

Dependent claims

A method, comprising:

generating a series of time-based clustering images for a plurality of contiguity preserved library fragments from a genome sample, wherein each time-based clustering image in the series is sequentially generated by:
introducing, to a flow cell, a respective sample including some of the contiguity preserved library fragments, wherein the some of the contiguity preserved library fragments are attached to a solid support or are attached to each other;
initiating release of the some of the contiguity preserved library fragments from the solid support or from each other;
amplifying the some of the contiguity preserved library fragments to generate a plurality of respective template strands;
staining the respective template strands;
and
imaging the respective template strands.

4 more elements

A method, comprising:

preparing a mixture including a plurality of contiguity preserved library fragments of a genome sample, the plurality of contiguity preserved library fragments being attached to solid supports or being attached to each other;
diluting the mixture to generate a predetermined number of dilution samples to be introduced to a flow cell;
and
generating a time-based clustering image for at least one of the contiguity preserved library fragments by:
introducing a first of the dilution samples including some of the contiguity preserved library fragments to the flow cell;
initiating release of the some of the contiguity preserved library fragments from the solid support or from each other;
amplifying the some of the contiguity preserved library fragments to generate a plurality of template strands;
staining the plurality of template strands;
and
imaging the plurality of template strands.

7 more elements

Dependencies are inferred from the claim text.

Time-based cluster imaging

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Time-based cluster imaging

Life of this patent

Abstract

Claims27