Compositions and methods for biosynthesis of terpenoids or cannabinoids in a heterologous system
AssigneeInmed Pharmaceuticals Inc.
InventorsVikramaditya G. YADAV, Protiva ROY, Sonal AYAKAR, Benson CHANG, Eric HSU
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Abstract
Provided herein are methods and compositions for producing cannabinoids and other metabolites in a host cell.
Claims48
A host cell comprising:
- a. an expression cassette comprising a promoter operably linked to a heterologous nucleic acid encoding a heterologous transporter or a functional fragment thereof, wherein the transporter is selected from the group consisting of a major facilitator superfamily (MFS) aromatic acid antiporter and an OprD family porin;
- and
- b. an aromatic substrate selected from olivetolate, divarinolate (DVA), or a metabolite, derivative, or decarboxylate thereof,
- wherein said host cell is capable of increased import of the aromatic substrate into the host cell as compared to a control host cell that lacks the expression cassette of a).
An expression cassette comprising a heterologous promoter operably linked to a nucleic acid encoding a bifunctional ispDE enzyme or functional fragment thereof.
An expression cassette comprising a heterologous promoter operably linked to a nucleic acid encoding a bifunctional ispDE, ispDF, or ispEF enzyme or a functional fragment thereof, preferably wherein the nucleic acid encodes a bifunctional ispDE enzyme or functional fragment thereof.
Dependencies are inferred from the claim text.